pLgw EcoDam-V5-RFC1

LOCUS       pLgw EcoDam-V5-RFC1              10221 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian DamID lentiviral vector for fusing gene of interest with 
            Dam-V5 using Gateway cloning .
ACCESSION   .
VERSION     .
KEYWORDS    pLgw EcoDam-V5-RFC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2598..2836
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2783..2804
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3731..3772
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3788..3912
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3937..3967
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4021..4701
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(4087..4106)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5021..5326
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5205..5224
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5367..5491)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             6022..6202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6228..6245)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6234..6458
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6504..6932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6591..6610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6801..6822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6941..6961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6946..7276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7127..7262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7189..7208
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7324..7371
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7390..7764
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7894..8015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7931..7950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7985..8004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8064..8080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8064..8080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8077..8099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8088..8104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8112..8142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8157..8178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8295..8312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8466..9054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8546..8565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9225..10085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9848..9867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10086..10190)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcccccgg gctgcaggaa
     2401 ttcagatctc ggccgcatat taagtgcatt gttctcgata ccgctaagtg cattgttctc
     2461 gttagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg
     2521 ttctcttgct gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcagtaccc
     2581 gggagtaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
     2641 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
     2701 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
     2761 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
     2821 caagaagaga actctgaata ctttcaacaa gttaccgaga aagaagaact cacacacagc
     2881 tagcgccacc atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct
     2941 gcttgatgat attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg
     3001 tgccgggtcg gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag
     3061 cgacctgatc agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc
     3121 acgcgagctg tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga
     3181 gttcaacaaa agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta
     3241 cggttacaac ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgttcggccg
     3301 ctacaaaaaa ccctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa
     3361 tgcctttttc tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt
     3421 cgtctattgc gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca
     3481 cacaaacagt tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt
     3541 tgagcgccat attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta
     3601 tcagcgcgca aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac
     3661 acgtaaaaag gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa
     3721 aaaaaccggt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgatcgattt
     3781 cgatcaccca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     3841 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     3901 ccagtcctat gggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3961 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
     4021 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     4081 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     4141 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     4201 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     4261 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     4321 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     4381 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     4441 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     4501 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     4561 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     4621 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggc ggggcgtaat
     4681 ctagaggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     4741 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     4801 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     4861 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     4921 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     4981 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     5041 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     5101 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtccc
     5161 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     5221 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     5281 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     5341 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     5401 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     5461 tttctcgttc agctttcttg tacaaagtgg ttcgatcgaa ccgcggtcta gactcgagac
     5521 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgcttgtgcc
     5581 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     5641 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5701 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     5761 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     5821 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     5881 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     5941 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     6001 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6181 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     6241 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6301 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6361 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6421 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6481 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6541 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6601 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6661 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6721 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6781 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6841 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     6901 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6961 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta
     7021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7201 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7261 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7321 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7381 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     7441 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     7501 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     7561 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     7621 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     7681 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     7741 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     7801 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7861 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8041 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8101 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8161 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8221 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8641 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9121 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9181 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9241 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9301 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9361 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9421 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9481 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9541 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9601 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9661 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9721 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9781 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9841 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9901 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9961 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10021 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10081 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10141 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10201 cgaaaagtgc cacctgacgt c
//